Muluneh Ademe Worku is an FLP awardee from Ethiopia from the Addis Ababa University. Muluneh’s PhD project is entitled “Mechanisms of Immune Suppression in Human Visceral Leishmaniasis and HIV co-infection”. Muluneh chose the Department of Internal Medicine, Division of Infectious Disease, University of Texas Medical Branch (UTMB), Galveston, Texas, USA under Peter C Melby for his PhD exchange project.

Muluneh Ademe Worku Report

Report of activities at the Department of Internal Medicine, Division of Infectious Disease, University of Texas Medical Branch (UTMB), Galveston, Texas, USA.

It is well recalled that I have been funded by FAIS fellowship award to support my subsistence expenses for the hands-on training on multi-parameter flowcytometry and immunofluorescence microscopy at University of Texas Medical Branch (UTMB), USA.

Once again, I am grateful for the fund. It helped me to learn a lot of laboratory trainings which I couldn’t find in Ethiopia. The document below includes the training report the invoice and some pictures at UTMB.

The aim of my trip to UTMB is two-fold. At one hand, I took hands-on training on different techniques including immunofluorescence staining and microscopy, multi-parameter flow cytometer staining, acquiring and analysis of results. On the other hand, I have done optimization of protocols. For instance, in my PhD study, leftover splenic aspirate sample will be used and processed for immunofluorescence microscopy. However, due to the low volume and cell number of the splenic sample, I have tried to check if cell block works and allows us to do immunofluorescence microscopy from sections as many times as necessary. Moreover, immunohistochemistry (IHC) protocols require microwave oven for antigen retrieval. However, there is no microwave in the field where I collect and process samples. In this regard, testing whether boiling in a water bath replaces the microwave antigen retrieval was necessary.
I would like to thank FAIS Legacy Project to give me this opportunity. I believe that we will keep in touch during the progress of my PhD work. I have tried to include the major activities I was involved during my stay at UTMB.

Sincerely,
Muluneh Ademe
Awardee of FAIS Legacy Project fellowship grant

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