Mohau Steven Makatsa is an FLP awardee from South Africa, University of Cape Town. Mohau’s project is entitled the “Characterization of Mtb-specific Th22 cells”. Mohau chose Dr. Mario Roederer’s lab at the Vaccine Research Center, NIAID/NIH, USA.

Mohau Steven Makatsa Report

Visit to Dr Mario Roederer’s lab, Vaccine Research Center, NIAID/NIH, USA

Courses attended: Advanced Flow Cytometry workshop

  • standardizing flow cytometry instrument (Calibration, Performance and Monitoring Standards)
  • 28 color flow cytometry panel development
  • Flow Data Analysis – Data Validation (FlowJo software) and Exploratory tools (FlowAI, FlowSOME, tSNE, UMAP, COMPASS etc.)
  • Flow cell sorting

Laboratory work:

  • Optimized flow panel to investigate cytokine production by different lymphocytes subsets
  • Perform whole blood assay on BCG vaccinated macaques
  • Run optimized flow panel on BCG vaccinated macaques